玉米灰斑病菌生理分化的寄主鉴定技术研究

Identification Technique of Physiological Differentiation of Gray Leaf Spot Pathogen

研究了玉米灰斑病菌致病性分化的鉴定方法、接种方法和环境条件对其的影响。结果表明:玉米苗期和成株期均可用于玉米灰斑病菌的抗性鉴定,利用成株期进行鉴定较好;利用自交系或品种均可以对玉米灰斑病菌的不同菌株进行抗性鉴定,从效果上看,利用自交系较好。对于玉米灰斑病菌来说,高粱粒灌心方法是抗性鉴定的首选方法。玉米灰斑病是属偏高温高湿类型的病害,在温湿度条件不能得到满足时,病害就难以完成侵染发病。土壤条件特别是N、P肥施用不均匀、不足或过多,均可对鉴定结果产生影响,在通常情况下,增施N、P肥能提高玉米对灰斑病的抗性。在一定光照强度范围内,光对鉴定结果的影响不明显。

This research studied the identification and inoculation methods of pathogenic differentiation of gray leaf spot（GLS） pathogen, and the effect of environmental conditions have also been considered. The results showed that the resistant identification of GLS pathogen in maize could be identified during the seedling and adult plant stage, the adult plant identification was better considering the easer inoculation. Identifying resistant identification using maize inbred was better than that of maize cuhivar. The method of that, inoculate sorghum seed with inocula into leaf whorl was the preferred method for resistant, GLS was prone to occur under high-temperature and high-humidity condition. The disease couldn＇ t infect when temperature and humidity wasn＇ t fitting. The concentration and spatial heterogene- ity of soil nutrition, especially, the content of nitrogen and phosphorus, all affect the infection ability of this disease. The high-nitrogen and high-phosphorus could improve the resistance of maize to GLS. To some extent, light intensity didn＇ t have significant influence on the infection of GLS.