小麦族猬草属和近缘属物种醇溶蛋白分析

Gliadin Analysis Among Species of Hystrix and Related Genera in Triticeae

利用酸性聚丙烯酰胺凝胶电泳(A-PAGE)对小麦族猬草属、赖草属、新麦草属、拟鹅观草属、披碱草属和Lophopyrum共6属19份材料进行醇溶蛋白遗传分析。结果表明:①19份材料共出现19种不同的醇溶蛋白图谱,共分离出56条带纹,多态性高达100%,说明属间和种间具有丰富的醇溶蛋白遗传多态性;②Hystrix duthieissp.duthiei与H.duthieissp.longearistata的亲缘关系较近,它们与H.patula的亲缘关系较远;Psathyrostachys juncea和Psa.huashanica亲缘关系较远;Lophopyrum elongatum和Lo.bessarabicum的亲缘关系较远;③含相似染色体组的物种能各自聚类在一起,它们具有较近的亲缘关系;④醇溶蛋白揭示的亲缘关系与形态学、细胞学研究结果基本一致,表明醇溶蛋白能够用于小麦族多年生物种系统亲缘关系分析。

The gliadin of 19 materials of Hystrix, Leymus, Psathyrostachys, Pseudoroegneria, Elymus and Lophopyrum in Triticeae were analyzed by using acid-polyacrylamide gel electrophoresis （A- PAGE）. The results show as follows： ①All the 19 materials have significant gliadin polymorphism. A total of 56 bands are separated, and the gliadin ploymorphism amounts to 100 %. It shows that there is plentiful polymorphism between these genera and species;②The relationship between Hystrix duthiei ssp. duthiei and H. duthiei ssp. longearistata is close, while they are remote to H. patula. The relationship between Psathyrostachys juncea and Psa. huashanica is remote. Lophopyrum elongatum is quite different from Lo. bessarabicum in gliadin patterns;③The species of those which contain similar genomes are clustered together, which suggests they have closer relationship;④The results of gliadin analysis are basically comparable with those obtained from studies on morphology and cytology, which shows that the gliadin patterns is usefulness in the systematic studies of those perennial species in Triticeae.