绿粘帚霉多菌灵耐药性菌株的筛选

Screening of Gliocladium virens Tolerant to Carbendazim

对培养7 d的绿粘帚霉(Gliocladium.virens)F051分生孢子悬浮液用15 W紫外灯,距离为30 cm处进行照射,并结合含药培养基培养,筛选得到了2株绿粘帚霉(G.virens)的多菌灵耐药性菌株。试验结果表明:紫外照射3min时,绿粘帚霉F051分生孢子的致死率达89.3%;当照射时间达到5 min时,孢子的存活率为0,故选择照射3min为本试验的最适诱变剂量;使F051致死的多菌灵最低终浓度为2μg/mL;诱变菌株与亲本菌株相比,抗药性提高了4倍,产孢能力更强,菌丝生长更为密集,这有利于生防真菌更快的抑制植物病原真菌的生长,减少农药的使用量,缓解化学防治对环境的压力。

The conidia of Gliocladium. virens （F051） which were cultured for 7 days were treated with the UV-light （15 W, 30 cm） radiation on PDA amended with carbendazim. Two Gliocladiurn. virens of resistant to carbendazirn were obtained by this way. The results show that： Irradiated for 3 min with the UV-light （15 W, 30 cm） radiation, the conidia lethal rate of Gliocladium. virens （F051） reaches 89.3%. When irradiated for 5 rain, all spores is to die and the survival is 0, then 3 rain irradiation is selected as the optimum mutation dosage and 2 μg/mL of carbendazirn concentration as Minimum lethal of F051 ; Compared with the parent strain, resistant level of mutants increases 4 times, their hypha grows more densly and their sporulation ability also improves, which is helpful to rapidly suppress plant pathogen and reduce the dosage of pesticide, and then reduce the pressure of environment caused by chemical control.