令箭荷花组织培养与植株再生的研究

Study on Tissue Culture and Plant Regeneration of Nopalxochia ackermannii

采用令箭荷花植株顶端幼嫩茎段作为外植体,以MS为基本培养基,采用不同激素配比分别诱导获得了愈伤组织、丛生芽和根。筛选出了适合令箭荷花植株再生的最佳激素浓度配比:6-BA 2 mg/L+NAA 0.2 mg/L适合愈伤组织的诱导,6-BA 1 mg/L+NAA 0.1 mg/L适合诱导丛生芽,IBA 0.2 mg/L有利于根的分化。

The top tender stalks of Nopalxochia ackermannii are used as explants in this paper. On the base of MS medium which are added different hormones, then the callus, adventitious buds and roots are induced respectively. The best density of hormones is selected from many kinds of combinations, in which the best one for inducing callus, buds and root are 6 - BA 2 mg/L ＋ NAA 0.2 mg/L, 6 - BA 1 mg/L ＋ NAA 0.1 mg/L and IBA 0.2 mg/L, respectively. The high efficient regeneration system of in vitro culture of Nopalxochia ackermannii is also established.