食品中金黃色葡萄球菌、单增李斯特氏菌和副溶血性弧菌的MPCR法检测

Detection of Staphylococcus Aureus, Listeria Monocytogenes and Vibrio Parahaemolyticus by Multiplex PCR

为快速检测食品中的金黄色葡萄球菌(SA)、单增李斯特氏菌(LM)和副溶血性弧菌(VP),根据各菌的相关基因设计引物,分别扩增金黄色葡萄球菌的耐热核酸酶基因-nuc、单增李斯特氏菌溶血素O上的hlyA基因和副溶血性弧菌的热稳定直接溶血素基因-tdh,建立一种MPCR快速检测食品中致病菌的方法,结果表明,三条特异性扩增片段分别为279bp、243bp和202bp,经DNA测序证明其序列与模板被扩增片段一致。该方法具有良好的灵敏性和特异性。

For establishing a rapid method to test staphylococcus aureus, listeria monocytogenes and vibrio parahaemolyticus in food, we designed three specific primers according to SA, LM and VP. The target gene was amplified from the three bacterium chromosomal DNA by using PCR technique. Electrophoresis products indicate there are three stips in 279bp, 243bp and 202bp. The target strips sequence analysis indicates nucleotide sequences are consistent with the templates, Proving the specimen is positive. The products reveal this method provides a specific, sensitive, and simple means for three bacterium mentioned above.