固态发酵灵芝多糖提取液中灵芝多糖的测定

The Determination of Ganoderma Polysaccharide in its extract of Glossy Ganoderma

本文研究了在蛋白质和还原糖含量比较高．颜色比较深的灵芝多糖提取液中，测定灵芝多糖含量的方法，结果表明，Sevage法与乙酸铅相结合除蛋白是一种理想的去蛋白方法，乙酸铅用量在0．26％～0．57％之间时，不仅可以排除蛋白质的干扰，而且乙酸铅对多糖测定也不会有影响；80％乙醇浓度可以使多糖完全沉淀下来。

This paper determined the content of Ganoderma polysaccharide extracted from glossy ganoderma which contained more protein and reducing sugar. The results indicated that combination of Sevage with lead acetate was a good removing-protein method. When the concentration of lead acetate was between 0.26% - 0.57%, the protein was removed and the lead acetate had no effect on the determination of polysaccharide. The polysaccharide was precipitated completely by using 80%ethanol.