黄芪减少砒石对大鼠氧化性损伤作用机制研究

Decreasing Oxygen Radical Damage by Taking Astragalus and Arsenolite together in Rats

[目的]探讨中药黄芪在减轻砒石致毒大鼠氧化性损伤及其对肝脏毒副作用的影响。[方法]50只SD大鼠随机分成5组,即(A)正常对照组,(B)砒石对照组(用10mg/kg),(C)砒石+黄芪(3.8g/kg)低剂量组熏(D)砒石+黄芪(7.6g/kg)中剂量组,(E)砒石+黄芪(15.2g/kg)高剂量组,灌胃给药1次/d,连续喂养12周后处死。检测增加黄芪治疗后的大鼠血清丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化酶(GSH-PX)、丙氨酸氨基转移酶(ALT)。普通石蜡切片并经苏木-伊红(HE)染色后用光学显微镜下观察肝组织的病理变化。[结果]砒石作用大鼠机体后,血清MDA升高、SOD降低,肝细胞损伤明显,而黄芪治疗组上述各值均较单纯砒石组改变(P<0.05),与正常对照无显著性差异,与单纯砒石作用大鼠相比,GSH-PX的活力均有明显升高,ALT值降低,差异有统计学意义(P<0.05)。[结论]黄芪在拮抗砒石致大鼠氧化性损害及其对肝脏毒性可能起到一定程度的作用。

[Objective] To investigate the possible mechanisms of oral administration of astragalus in decreasing liver toxic action and the oxidative damage in rats injured by arsenolite. [Methods] Fifty SD rats were randomly divided into five groups： group A （normal control group）, group B （arsenolite control group）, group C（arsenolite plus low dose of astragalus group）, group D （arsenolite plus middle dose of astragalus group）, group E （arsenolite plus high dose of ast.ragalus group）. All the determined medicines were given once a day. 12 weeks after feeding the animals were sacrificed. The level of serum malondialdehyde （MDA）, superoxide dismutase （SOD）, glutathione peroxidase （GSH-PX）, alanine aminotrans-ferase（ALT） were detected. The samples of liver were harvested to observe the pathological change with light microscopy. [Results] Compared with group B, the contents of MDA decreased, the activity of T-SOD and GSH-PX function improved markedly （P〈0.05 or P〈0.01） in group C, D and E, the difference was significant statistically. [Conclusions] Oral administration of astragalus membranccus and arsenolite together may decrease the oxygen radical damage and the liver injury induced by arsenolite in rats obviously.