摘要
以云南省农科院马铃薯研究中心选育的4个彩色马铃薯品系为试验材料，进行了叶片离体再生的研究。结果表明，DE02-24-24的叶片愈伤组织诱导的最佳培养基为MS＋6-BA2．5mg／L＋NAA0．2mg／L；DE02-24-39的叶片愈伤组织诱导的最佳培养基为MS＋6-BA2．0mg／L＋NAA0．1ms／L；JC02-27—1的叶片愈伤组织诱导的最佳培养基为MS＋6-BA2．0mg／L＋NAA0．1rag／L；JC02—27—2的叶片愈伤组织诱导的最佳培养基为MS＋6-BA3．0mg／L＋NAA0．15ms／L。愈伤组织的诱导率均可达到100％。愈伤组织诱导不定芽分化的最佳培养基分别为：DE02—24—24为MS＋6-BA2．0mg／L＋NAA0．1mg／L＋GA33．0ms／L；DE02-24-39为MS＋6-BA1．0ms／L＋NAA0．05mg／L＋GA32．0ms／L；JC02-27—1为MS＋6-BA1．0ms／L＋NAA0．05mg／L＋GA32．5ms／L；JC02-27-2为MS＋6-BA3．0mg／L＋NAA0．1mg／L＋GA32．0ms／L。其不定芽分化率分别为83．3％、100％、93.3％、100％。
The regeneration system of four cultivated colour popatoes in vitro was studied with leaves as explants, and the results showed that the optimal medium for callus induction from leaf discs was MS＋6-BA 2.5 mg/L ＋NAA 0.2 mg/L for ＂DE02-24- 24＂ ; MS＋6-BA 2.0 mg/L＋NAA 0.1 mg/L for ＂DE02-24-39＂ and ＂JC02-27-1＂ ; MS＋6-BA 3.0 mg/L＋NAA 0.15 mg/L for ＂JC02-27-2＂ .The optimal medium for adventitious bud differentiation from leaf-derived calluses was found to be MS＋6-BA 2.0 mg/L＋NAA 0.1 mg/L＋GA3 3.0 mg/L for ＂ DE02-24-24＂ ; MS＋6-BA 2.0 mg/L ＋NAA 0.1 rag/L＋ GA3 2.0 mg/L for ＂DE02-24- 39＂ ; MS＋6-BA 1.0 mg/L ＋NAA 0.05 mg/L ＋GA3 2.5 mg/L for ＂JC02-27-1＂ ; MS＋6-BA 3.0 mg/L ＋NAA 0.1 mg/L ＋GA3 2.0 mg/L for ＂JC02-27-2＂ . Adventitious bud differentiation rate of ＂DE02-24-24＂ , ＂DE02-24-39＂, ＂JC02-27-1＂, ＂JC02- 27-2＂ respectively 83.3%, 100%, 93.3%, 100%.
出处
《西南农业学报》
CSCD
2006年第B09期83-88,共6页
Southwest China Journal of Agricultural Sciences
基金
国家农业部“西部开发”重大项目（2005BA901A07）.
