摘要
目的：探讨几种理化因子对野葛悬浮培养细胞的生长和异黄酮化合物生成的影响。方法：采用高效液相色谱法和植物细胞培养的方法。结果与结论：随着蔗糖质量浓度的提高，野葛培养细胞生长量和异黄酮的累积均逐步增高；野葛细胞培养体系中pH维持在5．4—5．8，对异黄酮的形成最为有利；随着活性炭处理用量的增加，野葛培养细胞的干重和异黄酮积累急剧下降；与此相反，XAD-4处理促进野葛细胞的干重增长和异黄酮的积累。
Objective： To illustrate the effects of several physiochemieal factors on cell growth and isoflavone accumulation of Pueraria lobata cell suspension cultures. Method： High performance liquid chromatography and plant tissue culture were applied. Result and Conclusion： Cell growth and isoflavone accumulation were significantly stimulated in P. lobata cell suspension cultures by the increase of the sucrose concentration, Maintaining the pH value at the range over 5. 4 to 5. 8 was most suitable for isoflavone accumulation in P. lobata cell suspension cultures. Cell dried weight and isuflavone accumulation decreased sharply with the increase of the treated concentration of active carbon, while XAD-4 significantly stimulated cell growth and isoflavone accumulation.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2006年第19期1580-1583,共4页
China Journal of Chinese Materia Medica
基金
国家自然科学基金资助项目(30500051)
安徽省科技厅"十五"重大科技专项(01803025)
关键词
野葛
细胞培养
细胞生长
异黄酮
理化因子
Pueraria lobata
cell culture
cell growth
isoflavonoids
physiochemical factor
