应用组织芯片技术研究人类肺癌中EphB4、HIF-1α和IGF-Ⅱ表达的生物学意义

A study on biological significance of expression of EphB4,HIF-1α and IGF-Ⅱ in lung cancer through tissue microarray technique

目的应用组织芯片技术探讨人类肺癌中EphB4、HIF-1α和IGF-Ⅱ的表达情况以及三者表达的生物学意义。方法构建一个包含54例肺癌和10例正常肺组织的组织芯片蜡块,应用免疫组化SP法检测其EphB4、HIF-1α和IGF-Ⅱ蛋白的表达情况。结果病例组中EphB4、HIF-1α和IGF-Ⅱ蛋白的阳性率分别是44.4%、46.3%和42.6%,显著高于正常对照组(P<0.05);这三种蛋白的表达与肿瘤的大体类型、分化程度和TNM分期密切相关(P<0.05),而与患者性别、年龄、有无淋巴结转移无关(P>0.05)。三种蛋白的表达彼此之间存在显著正相关关系(P<0.01)。结论EphB4、HIF-1α和IGF-Ⅱ蛋白的表达可能与肿瘤的发生和恶性行为有关,三者之间存在着相互作用。

Objective To investigate the relationship among the expression of EphB4. HIF-laand IGF-Ⅱ in lung cancer and its biological significances. Methods Constructing a block with 54 lung cancer and 10 normal lung tissue on it. The expression of EphB4 .HIF-1α and IGF Ⅱwere detected in these specimens by using streptavidin-peroxidase （SP） immunohistochemical method, and 10 normal lung tissues as control. Results Among the 54 cases of lung cancer.the positive rate of EphB4 was 44.4 % 46. 3% of the tumors were positive for HIF-1α and 42. 6 %were positive for IGF-Ⅱ These three kinds of proteins expression were related to gross types,differentiations and clinical stages （ P〈0.05 ）. but not to histological classification, age.sex and lymphoid metastasis（ P〉0.05 ）. A highly positive correlation was observed between EphB4, HIF-1α and IGF- Ⅱ each other（ P〈0.01 ）. Conclusions Overexpression of EphB4, HIF-1α and IGF- Ⅱ may play an important role in the pathogenesis, progression and malignant degree of lung cancer. There are interaction between them each other.