奥硝唑对大鼠血胰屏障通透性的研究

Permeability of ornidazole through blood-pancreatic barrier in rats

目的研究奥硝唑对大鼠血胰屏障的通透性,为选择合适的抗微生物药物预防和治疗胰腺组织感染提供理论依据。方法给予大鼠口服奥硝唑20mg/kg,在规定时间点取样,用反相高效液相色谱法(RP-HPLC)测定胰腺组织和血清中药物的浓度。结果血清、胰腺组织中奥硝唑浓度在20m in时最高,分别为(13.24±2.01)mg/L,(18.34±3.26)mg/L。药物对血胰屏障的通透率(PR)在20m in以后一直>1。结论口服奥硝唑后,药物在胰腺中有良好的分布,对血胰屏障具有良好的通透性,值得向临床推荐用于预防和治疗胰腺感染。

OBJECTIVE To investigate the permeability of ornidazole through blood-pancreatic barrier in rats. METHODS Oruidazole （20mg/kg body weight） were fed to rats. At the given time points, the serum and pancreatic tissue samples were collected. The concentrations of oruidazole in the serum and pancreatic tissue were measured by HPLC. RESULTS The concentration - time profiles of oruidazole could be described as two - compartment model in rats; The peak concentrations in serum and pancreatic tissue were 13.24±2.01mg/L, 18.34±3.26mg/L at 20 min respectively. Then the concentrations decreased quickly in all of them. Concentrations in pancreatic tissue were higher than those in serum after 20 min and even at 360 min. The permeation ratio （PR） through blood - pancreatic barrier was higher continuously than 1 after 20 min. CONCLUSION Ornidazole can permeate blood - pancreatic barrier satisfactory, therefore it is worthy of being recommended for prevention and treatment of pancreatic infections.