基于APDL语言的结构优化设计

Design of Structure Optimization with APDL

以三杆桁架为例,介绍了运用APDL(Ansys Parameter Design Language)语言进行参数化建模及结构优化设计的过程,得出了参数的优化结果。该优化过程表明,运用APDL进行优化设计的方法避免了大量的重复有限元建模和前后处理操作,明显提高了产品的设计效率。

To construct eukaryotic expression vector encoding Mycobacterium tuberculosis Rv2450, the gene encoding Rv2450 protein was amplified by polymerase chain reaction（PCR） from genome of Mycobacterium tuberculosis H37Rv strain. After sequencing the gene was then subcloned into eukaryotic expression vector pCDNA3.1 （ - ）. The recombinant plasmid pCDNA-Rv2450 was transfected into P815 cells with liposome. The expression was detected with RT-PCR and indirect immuno fluensent. Rv2450 was cloned into pCDNA3.1 （ - ） correctly, and its expression was detected in P815 cells. Eukaryotic recombinant plasmids encoding Rv2450 were constructed successfully. The results established the basis for further study of the function of Rv2450.