大鼠脑出血后血管内皮生长因子和基质金属蛋白酶9的表达及七叶皂苷钠的影响

The Expression of Vascular Endothelial Growth Factor and Matrix Metalloproteinase-9 in Rats with Intercerebral Hemorrhage and the Response to Sodium Aescinate

目的 探讨血管内皮生长因子和基质金属蛋白酶9在脑出血后的作用及七叶皂苷钠的影响.方法 85只大鼠随机分为脑出血模型组（20）、治疗组A（20）、治疗组B（20）、假手术组（20）和正常对照组（5）,采用胶原酶复制大鼠基底节区脑内血肿模型,通过免疫组织化学动态测定不同时间点（6 h、24 h、48 h和96 h）鼠脑内血肿周围脑组织中血管内皮生长因子和基质金属蛋白酶9的表达;并同时检测七叶皂苷钠干预后两者在相应时间点的动态变化.结果 血管内皮生长因子和基质金属蛋白酶9在脑出血模型组出血后6 h表达明显增加,分别为12.67±1.50和9.27±1.28, 24～96 h显著增加且均处于高峰（P＜0.01）;在出血后6～96 h两者的表达呈正相关,相关系数为0.479（P＜0.01）.在七叶皂苷钠治疗组血管内皮生长因子、基质金属蛋白酶9的表达量明显受到抑制 （特别是治疗B组抑制效果更明显）,量效关系呈负相关其决定系数分别为0.107和0.083.结论 1.脑出血后血管内皮生长因子的表达水平提高可能有协同诱导基质金属蛋白酶9的表达作用.2.七叶皂苷钠可能通过抑制基质金属蛋白酶9和血管内皮生长因子的相关途径发挥抗脑水肿作用.

Aim To observe the expression of vascular endothelial growth factor （VEGF） and matrix metalloproteinase- 9 （MMP-9） in rat brain tissue in the border zone of the hematoma and explore the relationship among the expression of VEGF, MMP-9 and sodium aescinate after intracerebrul hemorrhage （ ICH）. Methods 85 heath SD rats were divided randomly for： intracerebral hemorrhage model group （20）, sodium aescinate treatment group A （20）, sodium aescinate treatment group B （20）, sham-operated group （20） and, normal contrul group （5）. Rat model of ICH was established by injection of physiological saline containing collagenase into the right caudate nucleus. Before and after interefered with sodium aescinate, the immunohistochemical methods were used to examine the expression of VEGF, MMP-9 in rat brain tissue at 6 h, 24 h, 48 h, and 96 h on ICH respectively. Results The amount of VEGF and MMP-9 were markedly increased at 6 h, respectively 12.67±1.50 and 9.27±1. 28 and reached the hightest on 24 - 96 h in the border zone after ICH （ P 〈 0.01 ）. There was a positive correlation with the expression of VEGF and MMP-9 after 6-96 h on ICH, coefficientcorrelationis0.479（P〈0.01）. The expression of VEGF and MMP-9 were inhibited transparently in sodium aescinate treatment group A and sodium aescinate treatment group B, especially in the latter and there was a negative dose-effect relationship, cecgicient of determination is 0. 107 and 0.083. Conclusions 1. The upregulated expression of VEGF might induce the expression of MMP-9 in the border zone after ICH in rats. 2. The antiencephaledema effects of sodium aescinate maybe related to change of the suppression of VEGF and MMP-9.