实时荧光PCR定性定量检测混合食用油脂中的花生油成分

Qualitative and quantitative detection of peanut oil in blend oil by real-time PCR

为鉴别鉴定花生油在食用油脂中的存在与否及其含量,研究了定性定量检测花生特异性基因片段的分子生物学检测方法。结果表明,生花生需加热处理后再提取DNA才能作为PCR扩增的模板,食用油脂中可提取出用于定性定量PCR的DNA,花生的Arah1基因是具有花生种属特异性的基因,采用实时荧光PCR方法检测Arah1基因片段可定性定量检测混合食用油脂中的花生油成分。

The identification and quantification of peanut oil in blend oil by using real-time PCR method was studied. Primers and Taqman probe was designed to detect Arachis hypogaea major allergen Arahl used as endogenous gene of peanuts in the assay. The results showed that DNA directly from the crude peanut and its derived oil were not suitable for PCR. The extracted DNA from the crude peanut and its derived oil which were heated could be employed as the template of PCR. Arahl gene was detected and it differentiated peanut from the other oil seads With real-time PCR method and specific primers and probe, the quantitative detection for the components of peanut oil in blend oil can be performed.