麝香百合子房离体培养的研究

Study on Ovary Culture in vitro of Lilium longiflorum

以麝香百合杂种系品种罗瑞拉(Lorina)的子房为试材,研究了外植体不同消毒方式、不同激素种类及其浓度、黑暗条件对离体培养的影响。结果表明:由于花瓣的包被,对花蕾不消毒也可达到百合子房离体培养的有效消毒效果。最佳愈伤组织诱导培养基为MS+2,4-D 0.5 mg/L+6-BA 1.0 mg/L,最佳分化培养基为MS+6-BA 1.5 mg/L+NAA 0.3 mg/L。黑暗条件下培养180 d后转入光照条件下培养可提高愈伤组织诱导率与分化率,并可有效防止外植体的褐化。

The ovaries of Lilium longiflorum cv. Lorina were used to study the effects of the different sterilization ways, different kinds and concentration of hormones, and dark condition on culture in vitro, As the results, not sterilizing also could achieve the effective sterilization effect. The optimum medium of callus induction was MS ＋ 2,4-D 0.5 mg/L ＋ 6-BA 1.0 mg/ L,and the optimum medium of differentiation was MS ＋ 6-BA 1.5 mg/L ＋ NAA 0.3 mg/L. After the ovaries were cultured in dark for 180 days and followed by in light, the callus induction and differentiation could be enhanced, and the browning of explants could be reduced effectively.