多药耐药基因相关蛋白在原发性肝癌中的表达及意义

Expressions of multi-drug resistance related genes in primary hepatocellular carcinoma and their clinical significance

目的探讨原发性肝细胞癌中耐药基因相关蛋白P-糖蛋白(P-gp)、DNA拓扑异构酶-Ⅱ(Topo-Ⅱ)、谷胱苷肽-S-转移酶(GST-π)的表达及其意义。方法应用免疫组织化学方法S-P法检测49例原发性肝癌组织、14例肝硬化组织及13例正常肝组织中P-gp、Topo-II、GST-π的表达。结果肝癌组三者表达均高于肝硬化组及正常组(P<0.05),肝硬化组与正常组之间两者表达差异无统计学意义。P-gp表达与肿瘤Edmondson分级呈负相关;Topo-II表达则与Edmondson分级和转移呈正相关。P-gp、Topo-II表达呈负相关。结论联合检测肝癌耐药基因相关蛋白P-gp、Topo-II、GST-π的表达有助于临床判断肝癌对化疗的敏感性及预后。

Objeodve To investigate the expression of P-gp and Topo-Ⅱ ,GST-π in hepatocellular carcimoma （HCC） and its relation to clinic pathological features. Methods Immunohistochemical staining was used to detect the expressions of P-gp,Topo-Ⅱ,GST-π in tissues of 49 cases of hepatocellular carcinoma, 14 cases of hepatic cirrhosis and 13 cases of normal liver tissues. Results The expressions of P-gp ,Topo-Ⅱ ,GST-π in HCC tissue were higher than those in hepatic cirrhosis and normal liver tissues, they had significant difference（ P 〈 0.05 ）. The expression of p-gp was negatively related to tumor Edmondson grade. The expression of Topo-Ⅱ was positively related to both Edmondson grade and metastasis. The expressions of P-gp was negatively related to those of Topo-Ⅱ. Conehtsionin The complex MDR is the main form of HCC multidrug resistance. Combined detection of MDR related proteins expression is helpful for predicting HCC sensitivity to chemotherapy and prognosis.