食管癌及癌前病变中NF-κB p65/p50蛋白的表达

NF-κBp65/p50 protein expression in esophageal cancer and precancerous lesions at high incidence area in Henan

目的探讨核转录因子NF-κB p65/p50蛋白在食管癌与癌前病变组织的变化特征及其生物学意义。方法采用免疫组化ABC法检测食管癌手术标本的NF-κB p65/p50蛋白表达状况并分析其特征。结果NF-κB p65/p50在正常食管鳞状上皮组织无表达,而在基底细胞增生(38%/32%)、间变(54%/46%)、原位癌(53%/47%)和鳞状细胞癌(69%/62%)均出现不同程度的阳性表达,并随病变进展,阳性表达率明显升高。而基底细胞增生与鳞癌之间表达率差异有显著性(P<0·05)。NF-κB p65/p50在食管鳞癌中表达有异质性。结论NF-κB p65/p50蛋白表达变化是食管癌和癌前病变组织常见的分子事件,可能在食管鳞癌癌变过程中起一定作用。

Purpose To evaluate the significance of NF-κB p65/p50 expression in esophageal cancer and precancerous lesions. Methods Immunohistochemistry （ABC） method was undertaken to determine the expression of NF-κB p65 and p50 proteins in esophageal carcinogenesis. Results The NF-κB p65 and p50 expression were not observed in normal esophageal epithelium, However, the different severity of NF-κB p65/p50 expression was observed in based cell hyperplasia （BCH, 38%/32% ） , dysplasia （54%/46%） , carcinoma in situ （53%/47%） and squamous cell carcinoma （SCC, 69%/62% ）. The positive rates for NF-κB p65 and p50 proteins increased with the lesion progression. Moreover, there were significant relationships among BCH, and dysplasia and SCC （P 〈 0. 05）, The heterogeneity of NF-κB p65 and p50 expression in esophageal squamous carcinoma was observed. Conclusions NF-κB p65 and p50 proteins are frequent molecular events in esophageal precancerous and cancerous lesions and may play an important role in esophageal carcinogenesis.