肝细胞癌中p504s的表达及临床意义

Expression and significance of the p504s in hepatocellular carcinoma by tissue microarray

目的探讨p504s在肝细胞癌中的表达和意义。方法收集134例肝细胞癌、122癌旁肝组织以及44例正常肝组织,应用组织芯片仪制备600点阵的组织芯片,采用EnVision免疫组化染色法对组织芯片进行p504s标记,并分析其表达与临床病理参数间的关系。结果600个点阵的组织芯片实际有效率为98·5%(591/600)。p504s在正常肝组织、癌旁、肝细胞癌中表达的阳性率呈依次下降趋势,分别为90·2%(37/41)、80%(96/120)和48·8%(61/129),其中肿瘤与癌旁,正常与肿瘤间比较差异有显著性(P<0·05)。p504s表达与肿瘤大小、血管侵犯、临床病理分期、分级和是否伴肝硬化等无关。结论p504s在肝细胞癌的发生过程中具有一定作用,可能成为一种新的肝细胞癌标记物。

Purpose To study the expression and significance of the p504s in hepatocellular carcinoma. Methods A tissue microarray consisting 600 chip, including 134 cases of hepatocellular carcinoma and corresponding adjacent nocarcinoma, 44 cases of normal liver was constructed. EnVision immnohistochemistry method was used to detect the expression of the p504s and its correlation to clinicpathological parameters was analysed statistically. Results 98.5% spots（591/600） were valid for final date analysis. Positive rate of p504s in normal liver, adjacent noncarcinoma and carcinoma, were 90. 2% （37/41） ,80% （96/120） and 48.8% （61/129） respectively. There were significant difference between cancer and adjacent noncancer, normal and cancer （ P 〈 0.05）. There were no significant relationship between its expression and maximal diameter, vascular invasion , clinicopathological stages, histological grade and cirrhosis. Conclusions p504s may be play a role in hepatocellular carcinogenesis, and may be potentially an important new marker of hepatocellular carcinoma.