小鼠黑色素移植瘤血管生成拟态CD31、MMP2、MMP9、Cathepsin D的表达

Expression of CD31、MMP2、MMP9、Cathepsin D on vasculogenic mimicry in the melanoma transplant tumor

目的动态观察CD31、MMP2、MMP9、Cathepsin D在黑色素移植瘤血管生成拟态形成（vasculogenesis mimicry，VM）中的表达。方法制作小鼠黑色素移植瘤动物模型，成瘤后每天随机抽取1只小鼠处死，共12天，获得肿瘤样本114例；HE染色、CD31、Cathepsin D、MMP2、MMP9免疫组化染色和PAS染色。结果在移植瘤形成的第1～8天，肿瘤组织内存在VM；第9～12天移植瘤中的VM被内皮依赖性血管替代；VM周围肿瘤细胞中CD31、MMP2阳性百分率高于远离VM区域的阳性百分率；内皮依赖性血管周围肿瘤细胞CD31阳性百分率与远离内皮依赖性血管区域的阳性百分率之间存在差别；VM周围肿瘤细胞MMP9阳性百分率高于内皮依赖性血管周围的阳性百分率；VM密度与VM周围肿瘤细胞CD31、Cathepsin D、MMP2阳性百分率之间存在相关；内皮依赖性血管密度只与内皮依赖性血管周围肿瘤细胞中CD31阳性百分率之间存在相关。结论移植瘤中VM是暂时性血液供应方式，渐被内皮依赖性血管替代；CD31、Cathepsin D、MMP2、MMP9均与黑色素移植瘤VM形成有关，其中CD31、MMP2作用尤其重要。

Purpose To study the effect of CD31 ,MMP2,MMP9,Cathepsin D on vasculogenic mimicry in the melanoma transplant tumor. Methods The mouse melanoma transplant tumor model was made with B16 melanoma cell suspending liquid. When the tumor appears, choosing one mouse each day randomly according to random number chart and kill it. To get 114 cases tumor specimens totally; The tumor specimens normally HE stain ,immunohistochemistry stain and CD31-PAS double stain. Counting the positive cell around the vasculogenic mimicry and far away from vasculogenic mimicry per 100 tumor cell; Counting positive cell around the endothelial-depended vessel and far away from the endothelial-depended vessel per 100 tumor cell. Results The vasculogenic mimicry and endothelial-depended vesselco-exist in the tumor tissue at the early phase of melanoma; From day 9 to day 12, this structure was replaced by the endothelial-depended vessel. The number of CD31 ,MMP2 positive cell around vasculogenic mimicry was more than that of far away vasculogenic mimicry. The ratio of CD31 positive cell around that of around endothelial-depended vessel . There were correlation between vasculogenic mimicry density and the number of CD31 ,MMP2 positive cells around vasculogenic mimicry. The density of endothelial vessel had correlation with the number of CD31 positive cell around endothelial-depended vessel. Conclusions The vasculogenic mimicry is one sort of temporary blood-providing pattern. CD31 ,Cathepsin D ,MMP2,MMP9 all take part in the development of vasculogenic mimicry and CD31 ,MMP2 play key role in this procession.