PTEN与VEGF在子宫内膜腺癌血管生成中作用的研究

Relationship between expression of PTEN and VEGF with angiogenesis in adenoendometrial carcinoma

目的研究子宫内膜腺癌组织中第10号染色体缺失的磷酸酶和张力蛋白同源物基因(PTEN)和血管内皮生长因子VEGF的表达与子宫内膜腺癌血管生成的关系。方法采用免疫组织化学SP法检测42例子宫内膜腺癌和20例正常子宫内膜(对照组)中PTEN和VEGF的表达,同时分析其不同表达状态下,子宫内膜腺癌组织中微血管密度(MVD)的变化。结果子宫内膜腺癌组和对照组PTEN蛋白表达阳性率分别为45.23%(19/42)和100%(20/20),两组比较差异有统计学意义(P<0.01)。子宫内膜腺癌组和对照组VEGF阳性率分别为69.05%(29/42)和10.00%(2/20),两组比较差异有统计学意义(P<0.01)。PTEN与VEGF的表达呈负相关(r=-0.611,P<0.05)。将子宫内膜腺癌分为4组,A组为PTEN阴性、VEGF阴性(n=8),B组为PTEN阴性、VEGF阳性(n=14),C组为PTEN阳性、VEGF阴性(n=10),D组为PTEN阳性、VEGF阳性(n=10)。4组平均MVD分别为30.71%、42.89%、25.46%和27.28%。B组显著高于其他3组,差异有统计学意义(P<0.05)。结论PTEN基因的失表达与子宫内膜腺癌的发生、发展密切相关。PTEN基因失活可能通过增加VEGF的表达来促进血管生成,导致子宫内膜腺癌恶性进展。

Objective To investigate the relationship of the expression of PTEN and VEGF with angiogenesis in adenoendometrial carcinoma. Methods Using immunohistochemical SP method, the expression of PTEN and VEGF was examined in 42 adenoendometrial carcinoma samples and 20 normal endometrium samples （control group）, and the microvessel density（MVD） was compared among different expression states of PTEN and VEGF in adenoendometrial carcinoma. Results The positive rates of PTEN in adenoendometrial carcinoma and control group were 45.23 % （19/42） and 100% （20/20）, and the difference between the two groups was significant （P 〈 0.01 ）. The positive rates of VEGF in adenoendometrial carcinoma and control group were 69.05 % （29/ 42） and 10.00% （2/20）, and there was significant difference between the groups（ P 〈 0.01 ）. And there was a negative correlation between PTEN and VEGF expression（ r = - 0.611, P 〈 0.05）. The adenoendometrial carcinoma group was subdivided into four groups. PTEN-negative and VEGF-negative cases as group A（ n = 8） ; PTEN-negative and VEGF-positive cases as group B（ n = 14） ; PTEN-positive and VEGF-negative cases as group C（ n = 10） ; PTEN-positive and VEGF-positive cases as group D（ n = 8）. The mean MVD of group A, B, C and D were 30.71% ,42.89% ,25.46% and 27.28% .The mean MVD of group B was significant higher than those of other three groups（ P 〈 0.05）. Conclusion The loss of PTEN expression is closely related to the carcinogenesis and development of the adenoendometrial carcinomal tumorigenesis. Inactivation of PTEN in adenoendometrial carcinoma may promote angiogenesis by increasing the expression of VEGF and thus induce tumor progression.