骨髓基质干细胞复合纳米羟基磷灰石/聚乳酸修复节段性骨缺损

Repair of Segmental Bone Defect by Fabrication of Tissue Engineering Bone with Bone Marrow Stromal Cells and Nano-hydroapatite/Polylactic Acid

目的了解骨髓基质干细胞(MSCs)复合纳米羟基磷灰石/聚乳酸(n-HA/PLA)构建的组织工程骨修复节段性骨缺损的可行性。方法选择30只新西兰白兔,制作15mm长的双侧桡骨节段性骨缺损模型,实验根据植入不同材料分为A、B组,A组分为A1组和A2组,A1组于动物左侧桡骨缺损区植入组织工程骨,A2组于右侧植入单纯材料。B组分为B1组和B2组,B1组于动物左侧桡骨缺损区植入自体髂骨,B2组右侧为空白对照。术后4、8、16周取材,行X线检查、组织学观察、计算机图像分析和生物力学测定。结果术后4、8、16周各组骨缺损区均有新骨生成,成骨量随着时间的推移而增加。经X线、组织学和生物力学评估,A1组与B1组比较未见差异(P>0·05),A1组或B1组分别与A2组或B2组比较,成骨能力依次为:A1或B1>A2>B2(P<0·001,P<0·05)。结论MSCs复合n-HA/PLA构建的组织工程骨可作为自体骨的替代材料修复节段性骨缺损。

Objective To study the practicability of repairing segmental bone defect by fabrication of tissue engineering bone with bone marrow stromal cells （MSCs） and nano- hydroapatite/polylactie acid （n- HA/PLA）. Methods The experimental model of 15 mm radial segmental defect was produced in 30 New Zealand white rabbits that were divided into group A and B according to transplant materials. Group A was further divided into subgroup A1 and A2. Tissue engineering bone was used to repair bone defect of left limbs in subgroup At, while simple material to repair defect of right limbs in subgroup A2. Group B was divided into subgroup BI and Bz. The iliac bone autograft was used to repair bone defect of left limbs, while defect of right limbs was used as vacant control group. When the sampies were harvested at 4, 8, 16 weeks postoperatively, a series of examination were carried out, including the roentgenographical, histomorphological, biomechanical and computerized graphical analysis. Results All of the defects which had been treated with implants exhibited increased new bone formation at 4, 8, 16 weeks postoperatively with time. There was no difference between the subgroup A1 and Bl （P 〉 0.05）, according to radiological, histomorphological and biomechanical evaluation. The comparison study showed that ability of new bone formation in four groups ranked as follows： A1 or B1 〉 A2 〉 132 （P 〈 0. 001, P 〈 0.05 ）. Conclusion The fabrication of tissue engineering bone with MSCs and n- HA/PLA could repair the segmental bone defect as the substitute of autograft.