吡格列酮对高糖高胰岛素诱导的心肌成纤维细胞增殖的影响

Effects of pioglitazone on cardiac fibroblast proliferation induced with high glucose and high insulin

目的利用体外培养的乳鼠心肌成纤维细胞,观察噻唑烷二酮(TZD)类药物吡格列酮对高浓度葡萄糖与高浓度胰岛素共同诱导的心肌成纤维细胞增殖的影响,进一步推测TZD类药物对糖尿病并发心肌肥厚的可能作用机制。方法以培养的乳鼠心肌成纤维细胞为模型分组给药后。利用结晶紫染色法测定细胞的数量;利用[3H]thym id ine标记法测定细胞DNA的合成;利用流式细胞仪分析细胞周期。结果吡格列酮可以抑制高糖高胰岛素诱导的心肌成纤维细胞数量、DNA合成及S+G2+M期细胞的百分比增加,并呈一定的剂量依赖性。结论吡格列酮能有效抑制高糖高胰岛素诱导的心肌成纤维细胞的增殖,这种作用可能是通过作用于PPARγ来实现的。

Aim To study effects of pioglitazone on cardiac fibroblast proliferation induced with high glucose and high insulin. Methods The neonatal rat cardiac fibroblasts were cultured and treated with different factors. The cellular number was determined by crystal violet uptake; the DNA synthesis was assayed with [ 3 ^H ] thymidine intake method ; the percentage of S ＋ G2 ＋ M in the cell cycle was determined by flow cytometry. Results Pioglitazone inhibited the cell number.DNA synthesis and the percentage of S ＋ G2 ＋ M inthe cell cycle of cultured cardiac fibroblasts induced with high glucose and high insulin. Conclusion Pioglitazone inhibits cardiac fibroblast proliferation induced with high glucose and high insulin and the effective mechanism of pioglitazone on cardiac fibroblast proliferation needs further study.