重组磷脂酶C的研究

Research of recombination phospholipase C

磷脂酶C是一类磷脂酰键的水解酶,在生物生命活动中起着第二信使的重要作用,能够抑制血小板的聚集作用,存在于原核生物和真核生物中,尤其是细菌中产生的磷脂酶C可以模拟真核生物中的作用而受到关注。该文叙述了磷脂酶C的克隆和表达、重构的工程菌株及重组磷脂酶C的纯化制备。同时,还简介了作者的研究,重组磷脂酶C可能进一步研究制成Ⅰ类抗血小板聚集作用新药。

Phospholipase C （PLC） is a kind of hydrolase for phosphoinositide （PI） , which plays a vital role in life activity as a second messenger. It can prohibit hematoblast from aggregating. PLC indwells the prokaryote and eukaryote, especially those existing in bacteria draw great attention since it can simulate the action in eukaryote. In this article, we describe the bas-ic mechanism of cloning, refolding, expressing of PLC, and the purification of recombinant PLC. At the same time, we report our study briefly, and we wish the recombinant PLC will be developed into a new medicine of anti - aggregation to platelet.