FasL基因转染NIH3T3细胞对BT325和U251胶质瘤细胞诱导凋亡作用的比较

Comparison of Apoptotic Effect between BT325 Human Glioblastoma and U251 Glioblastoma Inducted by FasL Gene Transferred NIH3T3 Fibroblast

目的比较FasL诱导2种胶质细胞瘤细胞凋亡作用的差异。方法用脂质体将FasL基因转染NIH3T3纤维母细胞,应用免疫组织化学染色法和RT-PCR法检测NIH3T3/FasL细胞目的基因的表达和转录,用Hochest 33342荧光染色法检验共培养体系中凋亡细胞种类,用TUNEL法检测共培养条件下胶质瘤细胞BT325和U251诱导凋亡作用的差异。结果NIH3T3/FasL细胞可有效表达FasL,与NIH3T3细胞(对照组)相比,有显著的诱导BT325和U251细胞凋亡的作用,对BT325胶质瘤细胞诱导凋亡的作用较U251胶质瘤细胞更为显著(P<0.05)。结论转染了FasL基因的NIH3T3细胞对BT325胶质瘤细胞诱导凋亡的作用比U251胶质瘤细胞更为显著。

Objective To compare the effects of FasL gene on the induction of apoptosis between two kinds of human glioma cells in vitro. Methods FasL gene was introduced into NIH3T3 fibroblast ceil by liposome reagents. FasL expressed by the NIH3T3/FasL cells was investigated with immunohistochemical staining and FasL mRNA was explored with RT-PCR. The ability of NIH3T3/FasL cells to induce apoptosis of BT325 and U251 glioblastoma co-cultured was assayed with Hochest 33342 fluorescent staining and TUNEL method respectively. Results FasL was expressed effectively in NIH3T3/FasL cells, and compared with the control group, the experimental group had significant effect on the induction of apoptosis of BT325 and U251 cells （P〈0.05）, the apoptosis of BT325 glioblastoma induced by NIH3T3/FasL was more significant than that of U251 glioblastoma（P〈0.05）. Conclusion FasL gene transferred NIH3T3 fibroblast has greater apoptosis induction effect on BT325 glioblastoma than on U251 glioblastoma.