基因芯片技术筛选2型糖尿病伴远端对称性多神经病变患者外周血单个核细胞差异表达基因

Gene microarray analysis of peripheral blood mononuclear cells in type 2 diabetes patients with distal symmetric polyneuropathy

目的:应用基因芯片技术筛选2型糖尿病伴远端对称性多神经病变患者外周血单个核细胞(PBMC)与不伴远端对称性多神经病变糖尿病患者以及正常个体相比的差异表达基因。方法:采用包含5 075个功能已知人类基因的cDNA芯片检测2名2型糖尿病并发远端对称性多神经病变患者(DSPN组),2名2型糖尿病不伴远端对称性多神经病变患者(DM组)以及2名年龄和性别匹配的正常个体(C组)PBMC基因表达谱,分析差异表达基因,并选择其中有差异表达的部分基因进行实时定量RT-PCR验证。结果:基因芯片技术筛选出22条差异表达基因,涉及细胞代谢与信号转导基因,原癌与抑癌基因,DNA合成和修复基因,离子通道与运输蛋白基因,DNA结合、转录和转录因子基因,细胞骨架组成基因等。其中上调基因4条,下调基因18条。实时定量RT-PCR测定下调基因中的AK1和FBXO7,与基因芯片技术结果一致。结论:2型糖尿病伴DSPN患者PBMC基因表达谱存在差异;DSPN可能涉及细胞代谢,信号转导和DNA合成等多个方面。

Objective,. To screen for the differentially expressed genes in peripheral blood mononuclear ceils （PBMCs） from type 2 diabetes patients with distal symmetric polyneuropathy （DSPN） by gene microarray. Methods： A 5 075 gene cDNA microarray was used to examine gene expression in PBMCs of 2 type 2 diabetes patients with DSPN （group 1）, 2 type 2 diabetes patients without DSPN （group 2） ,and 2 age- and sex-matched controls （control group）. The differentially expressed genes were identified and subjected to real-time RT-PCR analysis. Results： Twenty-two genes were found to be differentially expressed, including cell metabolism and signal transduction genes, oncogenes and anti-oncogenes, DNA synthesis and repair genes, ion channel and transport protein genes, DNA binding, transcription and transcription factor genes,and cytoskeleton genes. There were 4 genes up-regulated and 18 down-regulated. Real-time RT-PCR results of AK1 and FBXO7 were consistent with that of microarray analysis. Conclusion： Microarray expression profile of PBMC in type 2 diabetes patients with DSPN is differential. DSPN may he involved in ceil metaholism, signal transduction, DNA synthesis, etc.