改良处理猪主动脉瓣钙化的实验研究

An experiment of the calcification of reformed porcine aortic valve

目的研究新鲜猪主动脉瓣在体外钙沉积和幼兔的皮下种植实验中钙化的情况,初步探讨这几种瓣膜鞣制剂防钙化的作用机制。方法将新鲜的猪主动脉瓣经戊二醛(GA)、环氧氯丙烷(EC)直接处理,GA和乙醇联合处理,采用体外钙沉积和幼兔皮下种植两种钙化模型对新鲜猪主动脉瓣膜GA处理、EC处理、GA和乙醇联合处理进行体外钙沉积和体内钙化实验,通过光镜和等离子吸收光谱进行钙的定性和定量分析。结果各组瓣膜钙的含量较处理前增高,GA组钙的含量最高,EC组次之,其次是GA+乙醇处理组,新鲜瓣膜组钙含量最低,GA+乙醇处理组较单纯GA组钙的含量明显低(P<0·05)。在幼兔的皮下种植模型中,新鲜瓣膜组的钙含量最高,其次是GA组。结论用环氧氯丙烷直接处理生物瓣或GA处理后的生物瓣再用乙醇改性处理可以提高其抗钙化的能力,延长其使用寿命。

Objective To study the calcification of reformed porcine aortic valve with vitro experiment and rabbit subcutaneous embedding experiment and explore the mechanisms for preventing calcification. Methods The porcine aortic valves were treated with EC, GA ,and GA ＋ Ethanol respectively and then experimented to observe calcium deposition in vitro and in young rabbit subcutaneous embedding. Calcium content was measured with plasma absorption spectroscopy, and valve tissues were observed by light microscope. Results In vitro experiment revealed that calcification occurred in all groups, especially in the GA group. The calcium contents were obviously higher in the GA groups than in the fresh groups. The group treated with GA and ethanol were found with lower calcium contents than that of GA（P 〈 0. 05 ）. The calcium contents of the fresh valve groups were the highest in the young rabbit subcutaneous embedding experiment. Conclusion The anti-calcification effect and durability of prothetic valve will be improved through EC treatment or GA and ethanol treatment.