摘要
目的骨关节炎(osteoarthritis,OA)是最常见的一种慢性退行性骨关节疾病。近年的研究显示骨关节炎不但是一种退行性疾病,而且是一种关节内的炎症反应。通过检测OA软骨细胞CD80/CD86的表达情况,探讨软骨细胞在OA发病中的作用。方法收集10例膝关节骨关节炎患者软骨标本为病例组,同时收集8例股骨颈骨折患者软骨标本为正常对照组。进行软骨细胞培养和流式细胞技术,检测软骨细胞CD80/CD86的表达情况。进行统计学分析。结果病例组软骨细胞表达CD80的阳性细胞百分率(2.47%±0.59%)比正常对照组软骨细胞(0.94%±0.25%)高(P<0.01)。病例组软骨细胞表达CD86的阳性细胞百分率(1.71%±0.71%)比正常对照组软骨细胞(0.93%±0.47%)高(P<0.01)。结论CD80/CD86在骨关节炎患者软骨细胞中表达增加,做为抗原提呈细胞的标志分子,提示软骨细胞在骨关节炎中发挥抗原提呈作用。
Objective Osteoarthritis （OA） is the most common chronic joint disease. Studies have revealed that OA is not only a degenerative disease, but also an inflammatory disease. This study is going to investigate the roles of chondrocytes in OA by measuring the expression of CDS0/CD86 on chondrocytes. Methods Totally 18 specimens were obtained from 10 patients with OA who fulfilled the OA criteria of American Rheumatology Association formulated in 1986,8 patients with hip fracture as normal controls. The articular chondrocytes were cultured. The expression of CD80/CD86 was studied by flow cytometry. And the statistics results calculated by SPSS10.0 software show the statistics difference anlong the subgroups. Results The CD80 positive percentage of chondrocytes of OA group（2.47% ± 0.59% ） is higher than the control （0.94% ± 0.25% ） （ P 〈 0.01 ） .The CD86 positive percentage of chondrocytes of OA group（ 1.71 % ± 0.71% ） is higher than the control（0.93% ± 0.47% ） （ P 〈 0.01 ）. Conclusion CDS0 and CD86 are overexpressed on chondrocytes of OA patients. It indicate that chondrocyte fulfill the role of antigen-presenting cell in OA.
出处
《中国实验诊断学》
2006年第10期1167-1170,共4页
Chinese Journal of Laboratory Diagnosis
