三种方法测定血清总铁结合力比较

Comparisons of three measurement methods of total iron-binding capacity(TIBC) in serum

目的比较三种总铁结合力测定方法。方法对直接法、美国原装IBCT试剂盒及碳酸镁吸附法测定总铁结合力的精密度、相关性进行比较。结果直接法、美国原装IBCT试剂盒及碳酸镁吸附法CV值分别小于4.4、4.2、4.8%。三种方法比较,经方差分析显示,F=0.059,P=0.943,三种测定结果没有显著性差异。以美国原装IBCT试剂盒测定结果作为对比方法,直接法与碳酸镁吸附法线性方程分别为Y=0.967X+3.215,r=0.984;Y=1.019X-0.891,r=0.986。结论三种方法测定结果有较好的可比性;但直接法更简便,快捷,更能适应临床需要。

Objective To compare three measurement methods of TIBC in serum. Methods TIBC measured by Direct and full automated TIBC Assay, Iron-binding Capacity Test（IBCT） assay reagent and manual method by magnesium carbonate absorbent. Compare precision and correlation of three Methods. Results In three methods, we found the CV was lower than 4.4%, 4.2% and 4.8 % respectively. Compared three methods, the ANOVA shows： F = 0.059, P = 0.943, there are no significant different between three methods, we obtain the regression equation： Y1 = 0.967X ＋ 3. 215, r = 0.984; Y2 = 1. 019X - 0.891, r = 0.986（ Y1 is Direct and full automated TIBC Assay; Y2 is manual method by magnesium carbonate absorbent,X is Iron-binding Capacity Test（IBCT） assay reagent）. Conctusion A direct and full automated TIBC assay with an automated mulfipurposed analyzer is simple, rapid, accurate for clinical diagnosis.