肝病患者外周血中CD_4^+ CD_(25)^+ T细胞的变化与意义

Changes of CD_4^+CD_(25)^+T cell frequencies in the peripheral blood of patients with liver diseases

为了探讨肝病发病机制中CD4+CD25+T细胞的作用,本文采用全血裂解的方法得到单个核细胞,用流式细胞仪检测慢乙肝、肝炎肝硬化、重型肝炎患者外周血中CD4+CD25+T细胞的变化。实验测得重型肝炎、慢乙肝、肝硬化患者外周血中CD4+CD25+T细胞分别为7．53％、7．39％和6．47％,高于正常对照组的4．06％(P<0．01或0．05);而重型肝炎、慢乙肝、肝硬化患者外周血中CD4+T细胞分别为30．44％、38．12％和39．63％,均低于正常对照组的45．07％(P<0．01或0．05)。结果表明CD4+CD25+T和CD4+T细胞的变化可能与这些肝病的发生与发展有关。

In order to research the role of CD4^＋ CD25^＋T cell on the liver diseases, frequencies of CD4^＋ CD25^＋T cell were examined on peripheral blood mononuclear cells （PBMCs） by quadruple - colour flow cytometry. It was found that liver diseases patients show a high percentage of CD4^＋ CD25^＋T cell compared with healthy control. Chronic hepatitis B means （7. 39% vs. 4.06% , P 〈 0. 01 ） , liver cirrhosis means （ 6. 47% vs 4. 06%, , P 〈 0. 05 ） , severe hepatitis means （7. 53% vs 4. 06% , P 〈0. 01 ）,CD4^＋ CD25^＋T cell show a highly differentiated population and may play a role in liver diseases.