羊驼催乳素基因cDNA克隆及序列分析

Molecular Cloning and Analysis of Alpaca Prolactin Gene Sequences

实验通过克隆分析羊驼催乳素基因的部分序列,对羊驼催乳素基因的结构和功能进行初步探索和揭示。从GeneBank中已报到的脊椎动物催乳素基因保守区设计一对引物,采用Trizol法提取羊驼胎盘总RNA,利用RT-PCR技术扩增出长度约为510 bp的片断。测序后在NCB I工作平台中进行BLASTn同源性比较,得出结论:羊驼催乳素基因与已登录的哺乳动物催乳素基因同源性均超过85%,最高达97%。借助DNAstar分子生物学分析软件绘制了相关动物的遗传进化图,并对羊驼的种属地位进行了进一步验证。。

By cloning and analyzing the partial sequence of alpaca prolactin（PRL）, the purpose of this research is to qust for framework and function. According to conservative domain of the published sequence of prolactin gene of vertebrate in GeneBank, a pair of primers that can amplify alpaca PRL gene was designed and synthesized. The tatol RNA was extracted by Trizol. The fragments cDNA of about 510 bp was amplyfied by RT-PCR. The sequence was compared with published PRL genes in GeneBank by Blastn, the sequence showed hightest homology with PRL gene. All homology are over 85 % and the highest is 97 %. In virtue of DNAstar molecular biology analysis software, phylogenetic tree of different species animal PRL gene was protracted.