小儿急性淋巴细胞白血病HLA-A,B等位基因多态性研究

The polymorphism study of HLA-A,B alleles in children patients with acute lymphoblastic leukemia

目的研究北方汉族人群儿童急性淋巴细胞性白血病(ALL)患者与HLA-A,B等位基因多态性的遗传关联。方法采用聚合酶链反应-序列特异性寡核苷酸探针(PCR-SSO)方法,对197例北方汉族儿童ALL患者和5841例健康脐带血样本HLA-A,B等位基因多态性进行研究。结果在HLA-A,B等位基因中,儿童ALL患者的A11,A24,B40,B15,B56,B67,B27等基因的基因频率都显著高于正常人群,而HLA-B48基因的基因频率显著下降(P<0.05)。结论HLA-A11,A24,B15,B58,B67,B27等基因对儿童ALL患者有遗传易感作用,尤其是B40与ALL具有强相关性;而HLA-B48基因对儿童ALL患者有遗传拮抗作用。

Objective To study the genetic susceptibility of HLA-A, B alleles in children patients with acute lymphoblastie leukemia （ALL）. Methods HLA-A, B alleles polymorphism in 197 eases with ALL and 5841 normal umbilical cord blood controls were determined by PCR with sequenee speeifie olignueleotide pmbe（PCR-SSO）. Results The allele frequencies of All, A24, B40, B15, B56, B67, B27 were increased, while the allele frequencies of HLA-B48 were decreased in patients with ALL. Conclusion HLA-A11, A24, B15, B58, B67, B27 alleles seem to eootribute to the genetie suseeptibility for ehildren patients with ALL, especially B40 allele, while HLA-B48 to its genetic resistance.