多发性骨髓瘤的免疫表型特征

Immunophenotypic characteristics of multiple myeloma

目的研究多发性骨髓瘤(MM)细胞的免疫表型特征,以期为多发性骨髓瘤的诊断、预后判断和治疗监测提供可靠的依据。方法采用多参数流式细胞术,对55例MM患者和6例正常人骨髓细胞利用CD45PE-cy5/SS设门,在EPICSXL流式细胞仪上获取3×104个细胞,分析各群细胞CD10,CD20,CD38,CD56,CD19,CD138,CD34,CD13,CD7,CD33的表达。结果55例MM患者的CD138,CD38,CD56,CD20,CD19,CD13和CD33分别表达为100%,81.8%,20.0%,3.6%,10.9%和21.8%;CD45一般为阴性或弱阳性;其他标志均为阴性。结论多参数流式细胞术免疫表型分析在MM的辅助诊断、预后评估以及治疗监测方面是一项非常重要的技术。

Objective The main objective of this work was to investigate the immunophenotypic characteristics of mutiple myeloma. Methods We have examined the co-expression of CD19, CD20, CD34,CD13, CD38, CD45, CD56 and CD138 in marrow aspirates in 6 normal peoples and 55 patients with MM by flow cytometry. The analysis was based on at least 3×10^4 events. Results The percentage of MM cells ranged between 3.8 % and 63.2 % in 55 bone marrow aspirates of MM. The presence of CD138, CD38, CD56, CD20 CD19, CD13 and CD33 was expressed in 100 %, 100 %, 81.8 %, 20.0 %, 3.6 %, 10.9 % and 21.8 % respectively. The cells were negative or dim for CD45 antigens. The others were negative. Conclusion The immunophenotyping analysis of MM is a useful tool for diagnosis, further evaluating prognosis and detection of residual after treatment.