成年人脂肪源间充质干细胞治疗急性移植物抗宿主病后CD_8^+T细胞亚群的变化

Changes of CD_8^+ T cell subsets after treatment with adult adipose tissue-derived mesenchymal stem cells on acute graft-versus-host disease

目的初步探讨成年人脂肪源间充质干细胞(AMSC)治疗急性移植物抗宿主病(aGVHD)的分子机制。方法3例行异基因造血干细胞移植术后发生aGVHD的患者,以每1kg体重2×106个细胞剂量静脉输注AMSC;首先应用尼龙毛柱分离外周血T淋巴细胞,再经CD8磁珠分选出CD+8T淋巴细胞,应用流式细胞术检测发生aGVHD患者使用AMSC前后外周血CD+8T细胞亚群的变化。结果与输注AMSC前相比,输注AMSC后,CD+8T细胞中的CD+8CD2-8亚群显著上调,同时,患者的aGVHD得以有效控制。结论AMSC治疗aGVHD的作用机制可能与其上调CD+8CD2-8T细胞亚群有关,CD+8T细胞可能是AMSC作用的靶细胞。

Objective To investigate the molecular mechanism of treatment with adult adipose tissue-derived mesenchymal stem cells （AMSC） on acute graft-versus-host disease （aGVHD）patients. Methotis A dose of 2 × 10^6 AMSC per kg of the aGVHD patients‘ weight was given intravenously. T cells were harvested by using nylon column and CD8^＋ T cells were sorted by magnetic beads; the proportion of CD8^＋CD28^- T cells was assayed by fluorescence-activated cell sorter （FACS） in 3 cases with aGVHD following allogeneic hematopoietic stem cell transplantation. Results The data of FACS indicated that in the CD8^＋ T ceils,CD8^＋ CD28^- T cells were up-regulated significantly after AMSC transplant. At the same time, a complete remission of aGVHD disease was observed. Conclusion The molecular mechanism for the successful treatment with AMSC on aGVHD might be correlated with the up-regulation of CD; CD2^-8 T cells in vivo.