内质网在铝致神经细胞凋亡过程中的作用

Role of endoplasmic reticulum in aluminium-induced apoptosis

目的 探讨内质网在铝致神经细胞凋亡过程中的作用.方法 体外培养0～3 d新生大鼠的神经元细胞并用不同剂量氯化铝（AlCl3）染毒.（1）在光学显微镜和电子显微镜下观察神经元的凋亡和内质网的形态学改变;（2）测定凋亡相关蛋白Caspase和Ctyc在内质网的含量变化.结果 （1）光学显微镜和电子显微镜形态学观察发现了凋亡的典型形态学改变,电子显微镜下可观察到内质网的变形肿胀及脱颗粒现象.（2）凋亡相关蛋白Caspase的含量随染毒剂量的加大而上升,二者具有相关性;在染毒后可检测到Ctyc由内质网释放到胞浆的过程.结论 铝能诱导神经元凋亡,内质网在凋亡过程中发挥了重要的调控作用.

Objective To study the role of endoplasmetic reticulum in aluminium-induced apoptosis. Methods Neurons from 0-3 day newborn rats were cultured and treated with different concentration of aluminium chloride（ AlCl3 ）. （1）Morphologic changes of neurons and endoplasmic reticulum were observed under light and transmission electron microscope ; （2）Contents of apoptosis proteins, caspase and Ctyc in endoplasmic reticulum were measured. Results （1）Typical morphologic changes in neurons apoptosis and endoplamic reticulum were found under light and transmission electron microscope; （2）Active caspase-3 contents were significantly increased in M-treated groups comparing with the control group （ P 〈 0.01, r = 0.902）. There were significant higher CtyC contents in control group than those in Al-treated groups （ P 〈 0.05 ）. Conclusion A1 may induce neurons apoptosis, and endoplasmic reticulum plays an important role in the apoptosis progression.