分光光度法测定银杏萜内酯的含量

Spectrophotometric Determination of Total Terpenoids in Ginkgo Leaves

在弱碱性条件下,银杏萜内酯与Fe（Ⅲ）形成萜内酯-羟肟酸铁紫色环状配合物,最大吸收峰位于514 nm处,分光光度法测定萜内酯含量在45～450 μg/mL范围内服从比耳定律,标准曲线的回归方程为A=1.9316C＋0.0129,相关系数r=0.9994,测定结果与HPLC法对比实验一致.方法简便、快速,可用于银杏样品中萜内酯含量的测定.

A spectrophotometric determination of ginkgo terpene lactones is used. Purple circular complex between Fe（Ⅲ） and terpene lactones is formed in weak alkaline environment. Regression equation of terpene lactones is A = 1.9316C ＋ 0.0129 with a correlation coefficient of 0.9994. Beer＇s law is kept in the range of 45 ～ 450μg of per mL solution. Recovery and precision are relatively good. Result of the experiment is comparable to that of HPLC. The method is simple and fast, which is applied to the determination of terpene lactones in ginkgo sample.