乌贼墨对小鼠造血干细胞、粒-单系祖细胞及外周白细胞的影响

Effects of sepia on hematopoietic stem cells,granulocyte and monocyte progentior cells and peripheral WBC in mice

目的探讨乌贼墨对小鼠造血干细胞、粒-单系祖细胞及外周血WBC的影响。方法用不同剂量的乌贼墨灌胃正常小鼠、环磷酰胺(Cy)和辐射骨髓损伤模型小鼠,采用造血祖细胞体外培养方法和实验血液学技术,检测小鼠骨髓造血干细胞生成数(CFU-S)、粒-单系祖细胞集落生成数(CFU-GM)和外周血WBC数量。结果乌贼墨能够显著提高正常小鼠CFU-S,CFU-GM和外周血WBC数量,有效拮抗模型小鼠体内CFU-S,CFU-GM及外周WBC的降低,并显著促进模型小鼠上述各指标的恢复。结论乌贼墨具有显著的促进小鼠骨髓粒系造血作用。其作用机制可能通过调节机体的免疫机能,诱导机体产生粒/单核细胞集落刺激因子和多种细胞因子,促进造血细胞的增殖,并诱导造血细胞向粒单系细胞分化。

Objective To investigate the effects of sepia on stem cells, granulocyte and monocyte progentior cells and peripheral WBC in mice. Methods Different dosages of sepia were given to normal and model mice with hematopoietic system impairment respectively. The numbers of CFU-S, CFU-GM and peripheral WBC in normal and model mice were measured respectively with the method of hematopoietic progenitor cells cultured in vitro and the technique of experimental hematology. Results Sepia could enhance the number of CFU-S, CFU- GM and peripheral WBC in normal mice significantly, resist the decrease of CFU-S, CFU- GM, and peripheral WBC in model mice of hemapoiecsis impaired effectively and promote the restoration of those indices mentioned above in model mice significantly. Conclusion Sepia has significant effects on stimulating granulopoiesis in bone marrow in mice. The mechanism may be related to regulating immunological function and inducement GM-CSF and other sorts of cellular factors, which in turn promote the multiplication differentiation of CFU-S and CFU-GM.