中国南海海绵提取物-renierol抗痛风作用的实验研究

Antigout effect of renierol extracted from the marine sponge in the South China Sea

目的探讨中国南海海绵提取物renierol对黄嘌呤氧化酶的抑制作用及对小鼠高尿酸血症的影响和可能的作用机理。方法从海绵中分离提取renierol成分,采用尿酸生成法和超氧离子致NBT显色法测定黄嘌呤氧化酶活性,并进一步研究其作用机理。结果两种实验显示其对黄嘌呤氧化酶有明显的抑制作用,IC50分别为1.85和1.36μg.mL-1。结论该海绵中renierol成分能有效抑制黄嘌呤氧化酶。

Objective To investigate the inhibitive effect of renierol extracted from the marine sponge in the South China Sea on xanthine oxidase and the effect on mice hyperuricemia as well as its possible mechanisms. Methods Renierol was extracted from sponge and its antigoat effect was tested. The activity of xanthine oxidase was measured by using the generation of uric acid method and the NBT reduction induced by superoxide anions, then its possible mechanism was estimated. Results The two methods showed renierol could obviously inhibit the effect of xanthine oxidase with IC50of 1.85 and 1.36μg·mL^-1 , respectively. Conclusion Renierol extracted from the marine sponge can effectively inhibit the effect of xanthine oxidase.