国内养殖鱼类和进境鱼卵中传染性造血器官坏死病毒(IHNV)的检测及基因分析

Detection and Genetic Analysis of Infectious Haematopoietic Necrosis Virus (IHNV) from Cultured Fish and Imported Fish Larvae

用传染性造血器官坏死病病毒(IHNV)的反转录聚合酶链及该方法在来自国内养殖场的患病牙鲆和虹鳟及进口的匙吻鲟鱼卵中检测出IHNV,同时对扩增出的IHNV的DNA片段进行了序列分析。结果表明,从国内养殖的牙鲆和虹鳟中分离出的IHNV与IHNV的标准株RB-1株在序列上都具有98.1%的同源性,推导出在氨基酸序列上分别具有97%和99%的同源性。从进口匙吻鲟鱼卵中分离的IHNV与RB-1株在序列上都具有92.5%的同源性,推导出氨基酸序列有93%的同源性。系统发育树分析结果表明,从国内发病的养殖虹鳟中分离出的IHNV与进口匙吻鲟鱼卵中检测到的IHNV在进化上关系较为密切。

Reverse transcription polymerase chain reaction （RTPCR） and nested polymerase chain reaction （nested-PCR） were established for detectiing the infectious haematopoietic necrosis virus（IHNV）. The nucleotide fragments of 786 bp and 323 bp coding part of the viral nucleoprotein were amplified. IHNV were detected positively from cultured halibut, Paralichthys Oliuaceus and rainbow trout-from domestic fish farm with significant clinical signs and high mortalities. The homology of nucleotide sequence was 98. 1% between the amplified products of samples and IHNV reference strain, with the homology of 97% and 99% between the induced amino acids sequence. The samples from imported Mississippi paddlefish Polyodon spathala was found to be infected by IHNV and the similarities of nucleotide and amino acids sequence were 92. 5% and ,93% separately compared with reference strain of IHNV.