HPLC法测定缓释氟尿嘧啶植入后组织样的药物浓度

Determining the concentration of sustained release 5-fluorouracil implants in tissue by HPLC

目的 建立组织样中氟尿嘧啶的药物浓度HPLC测定方法。方法 HPLC测定，按中国药典2005版规定做方法学验证。结果 方法的主要指标如下：检测限为0．017μg·g^-1，日内精密度（RSD）约3．0％，日间精密度（RSD）约7．1％。方法 的回收率为92％～98％。结论 被测组织样的测定结果在0．14～40．85μg·g^-1。之间。该方法符合2005年中国药典关于生物样品分析方法的规定，是检测组织样品中氟尿嘧啶的药物浓度的可行方法。

Aim To establish a HPLC method for the determination of 5-fluorouracil in tissue. Methods 5-Fluorouracil was determined by HPLC according to Chinese Pharmacopoeia published in 2005. Results The main parameters of this method were as follows ： the limit of detection was 0. 017 μg · g^-1 ,the intra-day RSD was 3.0% ,the inter-day RSD was 7.1%. The recoveries were between 92% and 98.0%. Conclusion The method meets the criterion of China Pharmacopoeia 2005 for detecting biological specimen and is feasible for the determination of 5-fluorouracil in tissue.