籼稻不育系中A完善再生系统的研究

Efficient Plant Regeneration from in Vitro Culture of Indica Rice Zhong A (Oryza sativa L.)

以籼稻不育系中A的种子为外植体,探讨了培养基配方、外源激素、营养物等对其愈伤组织诱导和绿苗分化的影响。结果表明,在NB培养基中添加2.5 mg/L 2,4-D以及脯氨酸、水解酪蛋白、山梨醇等营养物有利于提高籼稻中A的愈伤诱导率,添加外源激素3.0 mg/L的6-BA和0.5 mg/L的NAA有利于提高其分化率,添加0.1 mg/L NAA可以使生根率达到100%。由此初步建立了籼稻中A的高效再生系统,为其遗传转化的顺利进行奠定了基础。

Factors affecting the callus induction and shoot differentiation of indica rice Zhong A, such as media, exogenous hormones and nutrient substances, etc. , were investigated. The results showed that high induction frequency of mature seeds derived embryogenic calli was initiated on NB medium supplement with 2. 5 ～ 3.0 mg/L 2, 4-D and nutrient substances such as praline, casein acid hydrolysate and D-sorbitol, high differentiation frequency was obtained on NB medium supplement with 3.0 mg/L 6 - BA and 0. 5 mg/L NAA, and 100 % rooting frequency was obtained on NB medium supplement with 0. 1 mg/L NAA. Therefore a high efficient regeneration system from in vitro culture of indica rice Zhong A was established for genetic transformation.