血管内皮生长因子在视网膜母细胞瘤中的表达及意义

Study on expression of VEGF in retinoblastoma

目的探讨血管内皮生长因子(vascularendothelialcellgrowthfactor,VEGF)在视网膜母细胞瘤中的表达及意义。方法用免疫组织化学方法对40例视网膜母细胞瘤患者的眼球石蜡块中的VEGF进行检测,同时以免疫组织化学方法检测CD105在血管内皮细胞中的表达,以此计算肿瘤的新生血管数量。结果VEGF主要表达于血管内皮细胞。VEGF高表达组的新生血管密度明显高于VEGF低表达组(P<0.01)。结论VEGF在视网膜母细胞瘤中的表达与肿瘤的新生血管形成有关,可能与肿瘤的转移有关。

[Objective] To study the expression of VEGF in retinoblastoma（Rb）. [Method] Immunohistochemistry（streptomycin avidin-biotinperoxidase complex SP） was used to detect the VEGF, CD105 protein expression in 40 cases of Rb. and calculate the microvessel density （MVD）. [Result] VEGF was expressed in endothelial cells, CD105 was also expressed in endothelial ceils. The microvessel density was significantly higher in VEGF highly expressed group than in the lowly expressed group （P 〈0.01）. [Conclusion] VEGF can promote angiogenesis in Rb and is correlated with tumor invasion and metastasis.