急性肺损伤早期氧自由基变化及参脉等中药的干预作用

Influence of chinese herbal on oxy-radicals in early ALI

目的比较不同中药通过抗氧自由基作用对急性肺损伤的影响。方法将实验动物随机分为10组,即:A1组:油酸+生理盐水注射液(盐水)后第6h组,A2组:盐水后第8h组;B1组:油酸+维生素C注射液(VC)后第6h组,B2组:油酸+VC后第8h组;C1组:油酸+参脉注射液(参脉)后第6h组,C2组:油酸+参脉后第8h组;D1组:油酸+葛根素注射液(葛根素)后第6h组,D2组:油酸+葛根素后第8h组;E1组:油酸+清开灵注射液(清开灵)后第6h组,E2组:油酸+清开灵后第8h组。用检测丙二醛(MDA)、超氧化物歧化酶(T-SOD)、谷光甘肽(GSH)和谷光甘肽过氧化物酶(GSH-PX)来评定氧自由基水平;用肺系数评定急性炎症和肺水肿程度;用右肺尖组织光镜病理学检查观察炎症浸润程度。结果①肺系数:6h组间无明显差异,8hE2组较A2明显增高;②MDA:6hC1组较其他各组明显增高,8hC2组较其他各组明显增高;③T-SOD:6h各组间无明显变化,8hE2组明显低于C2组;④GSH:6hD1组较其他各组明显增高,8hC2组较其他各组明显增高;⑤GSH-PX:各组间无明显变化;⑥光镜病理炎细胞浸润与肺泡充血水肿程度:A1、B1、C1、D1、E1组相似,A2>D2>C2>B2≌E2。结论VC、参麦、葛根素、清开灵均是可用的抗氧自由基药物,参脉降低MDA的作用略弱于其他药物;清开灵升高下T-SOD的作用较弱;葛根素和参脉升高GSH的作用较其他药物略强。光镜观察损伤后6h病理变化基本相似,8hVC、清开灵较参脉、葛根素干预组的损伤程度轻。

[Objective] To check and compare the different chinese herbal medicine influence on ALI through their function against oxy-radicals. [Methods] Divided the laboratory animals into 10 groups randomly, as these （the methods, injection, of the treatment to each member below were the same, here mentioned the different reagents for concision）： Group A1, 6 hrs after oleic acid ＋ NS; Group A2, 8 hrs after NS only; Group B1, 6 hrs after oleic acid ＋ VitC injection（VC）; Group B2, 8 hrs after VC; Group C1, 6 hrs after oleic acid ＋ Shenmai Injection; Group C2, 8 hrs after oleic acid ＋ Shenmai Injection; Group D1, 6 hrs after oleic acid ＋ Daidzein （Puerarin Injection）; Group D2, 8 hrs after oleic acid ＋ Daidzein（Puerarin Injection）; Group El, 6 hrs after oleic acid ＋ Qingkailing Injection; Group E2, 8 hrs after oleic acid ＋ Qingkailing Injection. Evaluated the level of free oxygen radical by detect MDA, T-SOD, GSH, and GSH-Px; evaluated the degree of acute inflammation and edema （lung） by lung coefficient; mention about the degree of the inflammation, it had to take the resort to the examination under light microscope pathologically, used the tissue from apex of right lung. [Results] ①Lung coefficient： no obvious differences among the ＂6 hrs Groups＂（A1,B1,C1,D1,E1） compared with the ＂8 hrs groups＂（A2, B2, C2, D2, E2）, and in the later groups, Group E2 was remarkable higher than Group A2; ②MDA： Group C1 increased mostly among ＂6 hrs groups＂, and so was Group C2 among ＂8 hrs groups＂;③ T-SOD： no marked differences among the ＂6 hrs Groups＂, and in ＂8 hrs groups＂, Group E2 was lower than Group C2 obviously; ④ GSH： Group D1 and C2 were the highest in ＂6 hrs groups＂ and ＂8 hrs groups＂ respectively; ⑤GSH- Px： no changing had been observed; ⑥ the degree of congestion and edema （pulmonary alveoli）： Group A1, B1, C1, D1, E1 were alike, and Group A2〉D2〉C2〉B2≌E2. [Conclusion] VC, Shenmai Injection, Daidzein（Puerarin Injection）, and Qingkailing Injection, all are available antioxyradical medicine. Some diversities of them four are： i Shenmai Injection is weaker than others in lowering MDA; ii when step up T-SOD, Qingkailing Injection is almost faint; iii DaidzeinfPuerarin Injection） and Shenmai Injection act a little stronger for GSH increase. The observation, 6 hrs after injury, are resemble on the whole; but if injured for 8 hrs, the groups treated with VC and Qingkailing Injection are milder than with Shenmai Injection and Daidzein （Puerarin Injection）.