纳米铁、纳米氧化锌和纳米氧化镁对小鼠血清乳酸脱氢酶和α-羟丁酸脱氢酶活性的影响

Effect of Nano-particle Iron, Zinc Oxide and Magnesia on Serum LDH and HBDH Activities in Mice

目的探讨纳米铁(Fe)、纳米氧化锌(ZnO)和纳米氧化镁(MgO)材料对小鼠血清乳酸脱氢酶(LDH)和α-羟丁酸脱氢酶(α-HBDH)活力的影响。方法分别以5g/Kg·wt剂量的纳米(Nano-)或微米(Micro-)粒径Fe、ZnO和MgO给小鼠一次经口灌胃,14d后处死取血分离血清分析LDH和α-HBDH活性变化。结果Nano-和Micro-Fe染毒小鼠血清LDH活力均比对照组明显降低(P<0.05),但Nano-Fe和Micro-Fe组小鼠血清LDH差异无显著性(P>0.05);对照、Nano-Fe和Micro-Fe三组小鼠血清α-HBDH差异无显著性(P>0.05)。Nano-MgO组小鼠血清LDH比对照组有所升高,但差异无显著性(P>0.05)。Micro-MgO组小鼠血清LDH比对照组和Nano-MgO组有明显升高(P<0.05);对照、Nano-MgO和Micro-MgO三组小鼠血清α-HBDH差异则无显著性(P>0.05)。两种粒径ZnO染毒组小鼠血清LDH和α-HBDH比对照组有显著性升高(P<0.05),其中Micro-ZnO组小鼠血清LDH和α-HBDH活力升高的更为显著(P>0.05)。结论Nano-Fe、Nano-ZnO和Nano-MgO材料对小鼠血清LDH及α-HBDH活性的影响与相应微米粒径材料有所不同。

Objective To explore the effect of oral administered nano- particle iron（Fe）, zinc oxide（ZnO）, and magnesia（MgO） powders on mice serum lactate dehydrogenase （LDH） and α-hydroxybutyrate dehydrogenase （α- HBDH） α-tivities. Methods The healthy mice were treated with micro- or nano - particle Fe, ZnO, and MgO powders at a single oral dose of 5 g/Kg body weight separately, After 14 days, the mice were sacrificed and the serum LDH and α- HBDH were evaluated. Results The serum LDH both in the nano- and micro- Fe groups was significantly lower than that in the control group. There was no significant difference in serum LDH between the nano - and micro- Fe groups. The serum LDH in the nano- MgO group was a little higher than that in the control group, but no significant （P〉0.05）. The serum LDH in the micro- MgO group was significantly higher than that in the control and nano - MgO groups. No significant difference was ob- served in serum α- HBDH among the control, nano- or micro - Fe groups and the control, nano - or micro - MgO groups, The serum LDH and α- HBDH in the nano- and micro - ZnO groups were significantly higher than that in the control group, the serum LDH and α- HBDH activities in micro - ZnO group were higher than that of the nano - ZnO group, Conclusion The effects of nano - Fe, nano - ZnO and nano - MgO on serum LDH and HBDH activities probably difference than that of micron material relatively.