高效液相色谱法测定化妆品中防腐剂

Determination of Preservatives in Cosmetics by HPLC

目的 建立化妆品中常用防腐剂对羟基苯甲酸酯类、苯甲酸、苯甲醇、卡松的测定方法。方法 采用高效液相色谱法，以乙酸铵（0．02mol／L）-甲醇为流动相，采用梯度淋洗，同时测定化妆品中7种常用防腐剂。结果各防腐剂在10～100μg／ml范围内呈良好线性关系（r〉0．999），平均回收率为91．5％～95．5％，相对标准偏差为1．3％～2．5％。结论 本法简便，快速，灵敏，准确，可用于化妆品中防腐剂的测定。

Objective To develop a HPLC method for determination of preservatives including parebens, benzoic acid, benzoic alcohol and kathon in cosmetics. Methods By using the HPLC method, mobile phase was ammonium acetate （0.02mol/L） - methanol and gradient elution was used to determine preservatives in cosmetics. Results Preservatives showed good linearity within the range of 10 - 100μg/ml （r〉0. 999）, average recovery waw 91.5% - 95.5%, RSD was 1,3 % - 2.5 %. Conclusion The HPLC method is simple, sensitive, and accurate and could be applied to determination of preservatives in cosmetic preparations.