子宫增重法检测壬基酚、双酚A雌激素样活性及其联合作用

Detection of estrogen-like activities for nonylphenol and bisphenol A and assessment of their joint effect with uterotropic assay

目的应用子宫增重法检测壬基酚（NP）、双酚A（BPA）的雌激素样活性并评价其联合作用。方法选择性未成熟雌性昆明种小鼠110只，体重10—13g，按体重随机分为11组：NP240mg／kg、120mg／k卧60mg／kg剂量组，BPA480mg／kg、240mg／kg、120mg／kg剂量组，联合240mg／kgBPA＋120mg／kgNP、120mg／kgBPA＋60mg／kgNP、60mg／kgBeA＋30mg／kgNP剂量组，阴性对照组、阳性对照组（苯甲酸雌二醇1．47μg／10g），皮下注射染毒3d，称子宫湿重，计算子宫重量系数。用金正均法评价联合作用的性质。结果NP和BPA可致子宫重量系数增大，呈剂量-效应关系（分别为r=0．8192，P〈0．01；r=0、6898，P〈0．01）；两者联合染毒致子宫重量系数增大，亦呈剂量一效应关系（r=0．5465，P〈0、01）。各染毒组与阴性对照组比较，差异有统计学意义（P〈0．05或P〈0．01），其联合作用性质在混合低剂量组呈协同作用（Q=1．41），混合中、高剂量组呈相加作用（Q=0.88，Q=0.87）。结论NP、BPA具有雌激素样活性，其联合作用性质在较低剂量呈协同作用，较高剂量呈相加作用。

Objective Try the uterotropic assay to measure the estrogen-like activities of nonylphenol （NP） and bisphenol A （BPA） and to assess their joint effect. Method One hundred and ten female Kunming mice were derided into 11 groups： control group, positive control group （estradiol 1.47μg/10 g）, three NP groups （mice were given 60 mg/kg, 120 mg/kg and 240 mg/kg NP, s.c. respectively）, three BPA groups （mice were given 120 mg/kg, 240 mg/kg and 480 mg/kg BPA, s.c. respectively）, and three joint effect groups （the mice were given 60 mg/kgBPA ＋ 30 mg/kgNP, 120 mg/kgBPA ＋ 60 mg/kgNP and 240 mg/kgBPA ＋ 120 mg/kgNP, s.c. respectively） for three days, then the uteri of mice were moved and weighed, and calculated the visceral coetScient of uteri, using JIN Zheng-jun＇s method to assess their joint effect. Result The exposure of NP and BP.A_ might induce the rise of uterus coefficient and be dose-dependent （ r = 0.819 2, P 〈 0.01 and r = 0.689 8, P 〈 0.01 respectively）. The joint administration of NP and BPA could also induce the rise of uterus coefficient and dose-dependent too （r = 0.546 5, P 〈 0.01）, there were significant or highly significant correlation compared with controls （P 〈 0.05 or P 〈 0.01）. The results revealed as well that the joint administration of these two compounds at low dose was shown as a synergistic effect （ Q = 1.41）, while at middle or high dose, it showed a additive effect （ Q = 0.88 or Q = 0.87）. Conclusion The results showed that NP and BPA have estrogen-like activities, their joint effects were synergistic or additive respectively at low dose level or at middle/high dose level.