蛴螬提取物对人乳腺癌MCF-7细胞增殖的影响及其机制探讨

Effect and mechanism of grub extract on cell cycle of human breast cancer cell line MCF-7

目的观察蛴螬提取物对人乳腺癌M CF-7细胞增殖的影响及其机制。方法采用M TT法检测蛴螬提取物处理过的人乳腺癌M CF-7细胞的生长抑制率,用免疫组化SP法检测用药前后增殖细胞核抗原(PCNA)、K i-67、细胞周期蛋白D 1(CyclinD 1)的表达改变,用流式细胞仪检测细胞周期的变化。结果用蛴螬提取物处理后的M CF-7细胞生长抑制率明显高于对照组,这种抑制作用呈浓度和时间依赖性;M CF-7细胞经蛴螬提取物处理后K i-67、PCNA、CyclinD 1表达均下降,且随着蛴螬提取物作用时间的延长,S期细胞比例明显升高。结论蛴螬提取物在体外对人乳腺癌M CF-7细胞株有显著的增殖抑制作用,其机制可能与下调CyclinD 1、K i-67、PCNA的表达有关。

[Objective] To observe the proliferation inhibitive effect of grub extract on human breast cancer MCF-7 cell line. [-Methods] MTT assay was used to measure the growth inhibition rate of human breast cancer MCF-7 cell line treated with the grub extract. The expression of PCNA,Ki-67 and CyclinD1 was detected by immunohistochemistry before and after exposure to the grub extract. FCM was used to assay the change of cell cycle. [Results] The growth inhibition rate of MCF-7 cells treated with the grub extract was significantly higher than that of control,and the inhibitive effect was time and concentration dependent. The expression of Ki-67, PCNA and CyclinD1 was down-regulated and the cells were cumulated in S phase. [Conclusion] The proliferation of human breast cancer MCF-7 cell line can be inhibited significantly by the grub extract in vitro. It is related with down-regulation of CyclinD1,Ki-67 and PCNA.