戊四氮点燃大鼠海马区NF-kB mRNA、IL-6 mRNA动态表达及地塞米松的影响

Dynamic expression of NF-kB mRNA, IL-6 mRNA in rats′ hippocampal zone ignited by PTZ and effect of Dexamethasone

目的探讨戊四氮慢性致痫大鼠海马区NF-kBmRNA、IL-6mRNA动态表达及地塞米松的抗痫机制。方法将Wistar大鼠随机分为对照组(C),非用药组(P),用药组(D),应用逆转录-聚合酶链反应(RT-PCR)对不同时间点(4、7、11、15d)海马区NF-kBmRNA、IL-6mRNA进行检测,同时根据行为学和脑电图确定大鼠痫性发作。结果用药组与非用药组NF-kBmRNA、IL-6mRNA海马区表达同对照组相比明显升高(P<0.05),并于点燃前达到高峰;同时用药组较非用药组相同时间点表达减少(P<0.05),且用药组点燃时大鼠痫性发作程度明显轻于非用药组,发作潜伏期也明显延长。结论NF-kB、IL-6参与了癫痫发病的整个过程,地塞米松通过抑制海马区NF-kBmRNA、IL-6mRNA表达起到抗痫作用。

[Objective] To discuss the dynamic expression of NF-kB mRNA, IL-6 mRNA in the hippocampal zone of chronic epilepsy rats caused by Pentylenetetrazol （PTZ）, and the anti-epileptic mechanism of Dexamethasone. [Methods] Wistar rats were randomly divided into three groups： the control group （C）, non-medical group （P） and medical group （D）. NF-kB, IL-6 in the hippocampal zone were detected using RT-PCR at different time and the seizures were determined by ethology and EEG. [Results] The expression of NF-kB mRNA, IL-6 mRNA in the hippocampal zone increased markedly comparing with that of C group （P 〈0.05） and reached the peak before ignited. While the expression of D group decreased comparing with P group （P 〈0.05）. [Conclusionl NF-kB, IL-6 participate during the whole course of epilepsy. Dexamethasone can restrain the expression of NF-kB mRNA, IL-6 mRNA in the hippocampal zone and play its anti- epileptic role.