厚皮甜瓜黄河蜜植株再生研究

Plant regeneration of Muskmelon cv.Huanghemi

利用厚皮甜瓜黄河蜜3号子叶及下胚轴进行植株再生实验研究．结果表明：子叶不定芽分化率高于下胚轴,适合子叶不定芽分化的培养基为MS+6-BA 1．5 mg/L+NAA0．2 mg/L,分化率为60%,每个外植体平均分化芽数9．4个．适合下胚轴不定芽分化的培养基是MS+6-BA 1．75 mg/L+NAA0．1 mg/L,分化率为9%,每个外植体平均分化芽数8．5个．对不定芽黑暗处理2天后用1/2 MS+IAA0．2 mg/L诱导生根,生根率达91．12%,平均根数为12条．200 mg/L的NAA对无根苗处理20 min后移入1/2河沙+1/2蛭石基质中,1/2 MS营养液浇灌,试管外生根率为52%．

This experiment was designed for plant regeneration from cotyledon and hypocotyls of muskmelon （Cucumis melo L.）cv. ＇Huanghemi＇. The results showed that the frequency of shoot regeneration of cotyledon explants was higher than that of hypocotyls explants, and the average shoot number regenerated from per cotyledon explants was more than that from per hypocotyls explants. The maximum shoot regeneration from cotyledon was obtained in MS containing 1.5 mg/L 6-BA and 0.2 mg/L NAA, the frequency was 60% and the average number of shoots per cotyledon was 9.4. The maximum shoot regeneration from hypocotyls was obtained in MS containing 1.75 mg/L 6-BA and 0.2 mg/L NAA, the frequency was 9% and the average number of shoots per hypocotyls was 8.5. After dark culture for 2 days, the roots was regenerated in 1/2 MS medium containing 0.2 mg/L IAA in the highest frequency, the frequency was 91.12% and the average number of roots per shoot was 12. Irrigation with 1/2 MS solution after socking in 200 mg/L NAA solution for 20 min was a good way for rooting out of the tuber, and the frequency was 52%.