摘要
用Ｑ－５２０型图象分析仪对食管正常粘膜上皮、癌旁非典型增生上皮及癌细胞分别测定每核Ａｇ－ＮＯＲｓ颗粒数、颗粒总面积，再用ＭＰＶ－３型显微分光光度计测定ＤＮＡ含量。结果显示：Ａｇ－ＮＯＲｓ颗粒计数在三组性质不同的细胞中，差异有显著性意义；Ａｇ－ＮＯＲｓ颗粒总面积和ＤＮＡ含量在三组细胞中差异有非常显著的意义。我们认为，核内Ａｇ－ＮＯＲｓ面积测定与ＤＮＡ定量分析，在食管癌变过程中，从不同角度了解细胞的生物学行为是一个非常有参考价值的定量指标，且有利于鉴别良、恶性病变。
Ag-NORs number and their total area in esophageal carcinoma(40 cases), dysplasia(24 cases) and normal epithelia(5 cases) were quantitatively counted by using the Q-520 Image Analysis System, while their DNA contents were measured by MPV-3 Morphometry. The results showed that both the Ag-NORs (mean count, P<0. 05; total area, P<0. 01) and DNA content(P<0. 01) of the above 3 groups were significantly different. It was suggested that the quantitative analysis of Ag-NORs and DNA content might provide some valuable data for the studies of biological behaviors and for the differentiation of the benignancy or malignancy of pathologic changes.
出处
《汕头大学医学院学报》
1996年第2期5-7,共3页
Journal of Shantou University Medical College
关键词
食管肿瘤
脱氧核糖核酸
显微分光光度法
esophageal neoplasms
DNA
Ag-NORs
quantitative analysis
microscope photometer
