摘要
利用透射电镜观察了放线菌酮体内诱导大鼠脾细胞凋亡的形态学变化,结果显示,腹腔注射放线菌酮4小时后,大鼠脾细胞发生凋亡,凋亡脾细胞核和胞质发生一系列形态学变化.主要表现为胞核染色质浓缩、重排、呈不同形态;多数凋亡脾细胞的粗面内质网增殖,凋亡小体形成,其线粒体也大量增加,或单个分布或团聚,肿胀、空泡化.结果提示,凋亡脾细胞的主要细胞器主动参与凋亡过程.
Using TEM, the splenocytes of the rats treated with cycloheximide in vivo were observed. The results revealed that rat splenocytes developed apoptosis 4 hours after the rats were injected intraperitoneally with cycloheximide. The apoptotic splenocytes occured a series of morphologic changes;The chromatin condensed and marginated the nuclei showed different appearances; the rough endoplastic reticulum lightly proliferated and apoptotic bodies formed. Their mitochondria increased also. They scattered individually or gathered in the cytoplasm. Gradually, the mitochondria swelled and vacuolized. The results indicated that the organellae of the apoptotic splenocytes take part in the apoptotic processes actively.
